Barbara Holshouser, PhD

Director, Center for Imaging Research
Professor, Radiology, School of Medicine
Medical Physicist, Department of Radiology, Loma Linda University Medical Center
E-mail: bholshouser@llu.edu

Research Interests

Dr. Barbara Holshouser, named as Director of CIR by the Dean of the School of Medicine, has over 35 years of experience in Radiology and imaging at LLU with past fellowships at Oak Ridge National Lab in radiological physics and Cal Tech in MRI imaging. As director, her main goal is to facilitate collaborations between basic science faculty and physicians to accomplish translational research using molecular imaging. Her main research interests include using advanced MR imaging to study central nervous system injury. Much of her research included the use of MR spectroscopy and MR spectroscopic imaging to characterize diseases such as cancer, Alzheimer’s and Parkinson’s.  She and colleagues used MR Spectroscopy and other advanced MR imaging techniques such as susceptibility weighted imaging, diffusion tensor imaging and perfusion weighted imaging to study traumatic brain injury for an NIH funded grant. Her most recent research interests involve the use of PET agents to study glioma, synaptic density and neuroinflammation.

Selected Publications

  1. Miriam E. Peckham ME, Dashtipour K, Holshouser BA, Kani C, Boscanin A, Kani K, Harder SL. "Novel Pattern of Iron Deposition in the Fascicula Nigrale in Patients with Parkinson’s Disease: A Pilot Study," Radiology Research and Practice, vol. 2016, Article ID 9305018, 7 pages, 2016. doi:10.1155/2016/9305018.
  2. Ashwal S, Tong KA, Ghosh N, Bartnik-Olson B, Holshouser BA.  Application of advanced neuroimaging modalities in pediatric traumatic brain injury. J Child Neurol. 2014 Dec;29(12):1704-17. doi: 10.1177/0883073814538504. Epub 2014 Jun 22. Review.
  3. Bartnik-Olson BL, Holshouser B, Wang H, Grube M, Tong K, Wong V, Ashwal S. Impaired Neurovascular Unit Function Contributes to Persistent Symptoms after Concussion: a Pilot Study. J Neurotrauma 2014 Apr 15.
  4. Hunter JV, Elisabeth A. Wilde EA, Tong KA, Holshouser BA. Emerging imaging tools for use with pediatric traumatic brain injury research. J Neurotrauma 2012 Mar 1;29(4):654-71. Epub 2011 Oct 17.
  5. Duhaime AC, Holshouser B, Hunter J, Tong K. Common Data Elements for Neuroimaging of Traumatic Brain Injury:  Pediatric Considerations. J Neurotrauma 2012 Mar 1;29(4):629-33. Epub 2011 Nov 2.
  6. Zechmann CM, Lagemaat MW, Fütterer JJ, Weiland E, Lu J, Holshouser BA, van Hecke P, LeMort M, Schlemmer H-P, Villeirs GM, Barentsz JO, Roell SO, Heerschap A, Scheenen TWJ. Reproducibility of 3D 1H MR spectroscopic imaging of the prostate at 1.5T. J Magn Reson Imaging. 2012 Jan;35(1):166-73. doi: 10.1002/jmri.22827. Epub 2011 Sep 29.
  7. McAuley G, Schrag M, Barnes S, Obenaus A, Dickson A, Holshouser B, Kirsch W. Iron quantification of microbleeds in postmortem brain. Magn Reson Med. 2011 Jun;65(6):1592-601.
  8. Ashwal S, Wycliffe ND, Holshouser BA.  Advanced neruoimaging in children with nonaccidental trauma. Dev Neurosci. 2010;32(5-6):343-60.
  9. Colbert C, Holshouser BA, Aaen G, Sheridan C, Oyoyo U, Kido D, Ashwal S. Value of Cerebral Microhemorrhages detected with Susceptibility Weighted MR Imaging for prediction of Long-term Outcome in Children with Non-accidental Trauma. Radiology. 2010 Sep;256(3):898-905.
  10. Holshouser BA, Tong KA, Ashwal S, Oyoyo U, Ghamsary M, Saunders D, Shutter L. Prospective longitudinal proton magnetic resonance spectroscopic imaging in adult traumatic brain injury. JMRI 2006;24:33-40.
  11. Holshouser BA, Tong KA, Ashwal S. Proton magnetic resonance spectroscopic imaging detects diffuse axonal injury in pediatric brain trauma. AJNR Am J Neuroradiol 2005;26:1276-1285.
  12. Holshouser BA, Babikian T, Freier MC, Burley T, Tong KA, Ashwal S. Proton spectroscopy predicts neuropsychological outcome after pediatric traumatic brain injury. Abstract and Poster presentation at International Society of Magnetic Resonance in Medicine, 13th Scientific Meeting, Miami Beach, FL USA, May 9-13, 2005.
  13. Holshouser BA, Ashwal,S, Shu, S, Hinshaw, DB Jr. Proton MR Spectroscopy in Children with Acute Brain Injury: Comparison of Short and Long Echo Time Acquisitions. J Magn Reson Imaging 11:9-19, 2000.
  14. Holshouser BA, Ashwal S, Luh GY, Shu S, Kahlon S, Auld KL, Tomasi LG, Perkin RM, Hinshaw DB Jr. 1H-MR Spectroscopy after Acute CNS Injury: Outcome Prediction in Neonates, Infants and Children. Radiology, 1997; 202:487-496.

Umesh Gangadharmath, PhD

Technical Director, Radiochemistry, Center for Imaging Research
Assistant Professor, Radiology, School of Medicine
Department of Radiology, Loma Linda University Medical Center
E-mail: ugangadharmath@llu.edu

Biography and Research Interests

Dr. Umesh Gangadharmath is an Assistant Professor of Radiology and Basic Sciences at Loma Linda University Medical Center, Loma Linda, CA.  Umesh directs the radiochemistry lab for preclinical and clinical research in molecular imaging and is an important team member of the Center for Imaging Research.  Dr. Gangadharmath has extensive radiochemistry experience both in academic and commercial settings.  His main research interests are in designing and synthesizing novel radiopharmaceuticals for use in positron emission tomography (PET) imaging of oncology, cardiology and neurological targets. He has worked on markers for hypoxia (18F-HX4), apoptosis (18F-CP18) and markers for Tau (18F-T808/T807(AV1451)).  He has extensive experience in designing novel molecules for PET Imaging.  He has many years of experience in process optimization, quality control, technology transfer, regulatory filling, FDA audit and responses.  Dr. Gangadharmath has established PET tracer manufacturing sites globally (US, EU and Asia) to support PET imaging-based clinical trials.  Dr. Gangadharmath is a Participating member of the Editorial Advisory Board for Radiochemical Syntheses: Radiopharmaceuticals for Positron Emission Tomography (publisher: Wiley) and is currently serving as a member of United States Pharmacopeia (USP) Radioactive Drugs Expert Panel.  Dr. Gangadharmath has authored many publications and patents.

Selected Publications

  1. Noninvasive molecular imaging of apoptosis in a mouse model of anthracycline-induced cardiotoxicity. Circ Cardiovasc Imaging 2015 Feb;8(2):e001952. H. Su, N. Gorodny, L. Gomez, U. Gangadharmath, F. Mu, G. Chen, J. Walsh, K. Szardenings, H. Kolb and B. Tamarappoo.
  2. Atherosclerotic plaque uptake of a novel integrin tracer ¹⁸F-Flotegatide in a mouse model of atherosclerosis. J Nucl Cardiol 2014 Jun 14;21(3):553-62. H. Su, N. Gorodny, L. Gomez, U. Gangadharmath, F. Mu, G. Chen, J. Walsh, K. Szardenings, D. Berman, H. Kolb and B. Tamarappoo.
  3. [18F]T807, a novel tau positron emission tomography imaging agent for Alzheimer's disease.  Alz. & Dementia. Alzheimers Dement 2013 Nov 12; 9(6):666-76. C. Xia, J Arteaga, G. Chen, U. Gangadharmath, L. Gomez, D. Kasi, C. Lam, Q. Liang, C. Liu, V. Mocharla, F. Mu, A. Sinha, H. Su, K. Szardenings, J. Walsh, E. Wang, C. Yu, W. Zhang, T. Zhao and H. Kolb.
  4. Evaluation of [18F]-CP18 as a PET Imaging Tracer for Apoptosis.  Journal of Nuclear Medicine. In Press Mol Imaging Biol 2013 Dec;15(6):739-47. H. Su, G. Chen, U. Gangadharmath, L. Gomez, Q. Liang, V. Mocharla, K. Szardenings, J. Walsh, C. Xia, C. Yu and H. Kolb. 
  5. In Vitro and In Vivo Evaluation of the Caspase-3 Substrate-Based Radiotracer [18F]-CP18 for PET Imaging of Apoptosis in Tumor. Molecular Imaging And Biology. Mol Imaging Biol 2013 Dec;15(6):748-57. C. Xia, G. Chen, U. Gangadharmath, L. Gomez, Q. Liang, F. Mu, V. Mocharla, H. Su, K. Szardenings, J. Walsh, T. Zhao and H. Kolb.
  6. A New Class of Highly Potent Matrix Metalloproteinase Inhibitors Based on Triazole Substituted Hydroxamates: (Radio) Synthesis and in Vitro and First in Vivo Evaluation.   J. Med. Chem., 2012, 55 (10), 4714–4727 V. Hugenberg, H.-J. Breyholz, B. Riemann, S. Hermann, O. Schober, M. Schäfers, U. Gangadharmath, V. Mocharla, H. Kolb, J. Walsh, W. Zhang, K. Kopka and S. Wagner.
  7. A Highly Selective and Specific PET Tracer for Imaging of Tau Pathologies.  J. Alz. Dis. 2012, 31, 1–12  W. Zhang, J. Arteaga, D. Cashion, G. Chen, U. Gangadharmath, L. Gomez, D. Kasi, C. Lam, Q. Liang, C. Liu, V. Mocharla, F. Mu, A. Sinha, K. Szardenings, E. Wang, J. Walsh, C, Xia, C. Yu, T. Zhao and H. Kolb.
  8. Radiochemical Syntheses: Radiopharmaceuticals for Positron Emission Tomography: Radiosynthesis of 3’-Deoxy-3’-[18F]Fluorothymidine ([18F]FLT). H. Kolb, H. Padgett, S. Zigler, J. Patanella, F.  Mu, U. Gangadharmath, V. Mocharla, P. J. H. Scott and J. Walsh.
  9. Inverse 1,2,3-Triazole-1-yl-ethyl Substituted Hydroxamates as Highly Potent Matrix Metalloproteinase Inhibitors: (Radio)synthesis, in Vitro and First in Vivo Evaluation. J Med Chem 2013 Sep 16;56(17):6858-70. V. Hugenberg, B. Riemann, S. Hermann, O. Schober, M. Schäfers, K. Szardenings, A. Lebedev, U. Gangadharmath, H. Kolb, J. Walsh, W. Zhang, K. Kopka and Stefan Wagner.
  10. Radiochemical Synthesis, Volume: Further Radiopharmaceuticals for Positron Emission Tomography and New Strategies for Their Production 2.
  11. Radiosynthesis of [18F]Flotegatide ([18F]RGD‐K5). H. Kolb, F. Mu, U. Gangadharmath, V. Mocharla, Z. Zhu, A. Chaudhary and J. Walsh.

Selected Patents

  1. Nitro-Imidazole Hypoxia Imaging Agents.  EP2132200A2, WO2008124651. H. Kolb, J. Walsh, U. Gangadharmath, F. Karimi, H. Padgett, D. Kasi, Z. Gao, Q. Liang,L. Collier, B. Duclos and T. Zhao.
  2. Application of Staudinger Ligation in PET Imaging.  US2011/030325, WO/2011/123444. D. Kasi, U. Gangadharmath, J. Walsh and H. Kolb.
  3. Synthesis of 18F-labeled Tracers in Hydrous Organic Solvents. US2011/031681, WO/2011/127345.U. Gangadharmath, J. Walsh and H. Kolb.
  4. Imaging agents for detecting neurological disorders.  US8691187 B2. K. Szardenings, W. Zhang, D. Kasi, A. Sinha, U. Gangadharmath and J. Walsh.
  5. Formulation, Apparatus and Method for Stabilizing Radiopharmaceuticals.  US2011/047717, WO/2012/021882J. Walsh, H. Kolb, V. Mocharla, F. Mu and U. Gangadharmath.
  6. Novel Substrate Based PET Imaging Agents.  US 2010/0074843 A1. H. Kolb, J. Walsh, K. Chen, U. Gangadharmath, G. Chen, V. Mocharla, D. Kasi, P. J. H. Scott and Q. Liang.
  7. Imaging Agents For Detecting Neurological Dysfuntion. US 8,932,557 B2. G. Chen, U. Gangadharmath, D. Kasi, A. Sinha, W. Zhang, K. Chen and V. Mocharla.
  8. PSMA imaging agents WO 2013028664 A1 E. Wang, H. Kolb, K. Szardenings, C. Liu, J. Walsh, G. Chen, A. Sinha, D. Kasi, C. Yu, U. Gangadharmath, W. Zhang, T. Zhao, Vs. Mocharla.