Cellular Biology | School of Medicine

Agilent Seahorse XFe24 Analyzer

The Agilent Seahorse XFe24 Analyzer is a microrespirometer specialized for making real time measurements of Oxygen Consumption Rate (OCR) and Proton Efflux Rate (PER) in cultured cells or whole tissues mounted in 24-well culture plates. Measured values of OCR and PER can be used to calculate glycolytic flux and/or rates of ATP synthesis and is uniquely well-suited for studies of mitochondrial respiration and substrate utilization.

Bruker Elexsys E500 CW-EPR SuperX

The Bruker Elexsys E500 CW-EPR SuperX system is a Continuous Wave Electron Paramagnetic Resonance spectrometer that employs a Dual Gunn source to create a super high Q cavity with high-power, high sensitivity, and ultra-low noise. The data capture paradigm displays up to 256,000 points on the field axis, and 65,536 increments on the transient time axis with an ultra-high-resolution field controller and digital rapid free scan module that together enable reference free spin counting during stationery or time resolved experiments. This instrument is ideally suited for measurements of electron behavior in general chemistry, material science, physics and life science experiments.

Leica CM3050 S Cryostat

The Leica CM3050 S Cryostat offers precise sectioning with accurate and variable specimen orientation and feed at highly stable cryochamber temperatures between 0 to –40°C. This cryostat includes a system to count sections that can be reset for trimming at different levels. In reverse mode, the section counter enables planning for a preselected number of sections at any section thickness. The maximum specimen size is 40 mm X 55 mm and cutting speed can range from 0.1 mm to 170 mm per second.

Leica LMD7

Leica LMD7 is a laser capture microdissection microscope that can isolate cellular Regions of Interest (ROI) from slide-mounted sections. The laser beam originates overhead of the sample and uses one of five different cutting modes to cut perpendicular to the tissue with precision and speed. Laser power, aperture size, speed, head current and pulse frequency are all adjustable. This system can isolate the entire area of a tissue section, a single cell, or as little as subcellular structures such as chromosomes. Captured subsamples can be used for transcriptomic analyses, proteomics, metabolomics, and next generation sequencing.

MACSQuant Analyzer 10 Flow Cytometer

The MACSQuant Analyzer 10 Flow Cytometer offers versatility and power for investigation of whole cell signaling pathways, separation of rare cells from mixtures, and analysis of cell manufacturing efficiencies. The onboard optics include three spatially separated laser excitation wavelengths and 10 emission detectors, each of which are optimized with spectrally matched PMT’s for all channels that can measure up to 15,000 events per second. The fluidics system requires a minimal uptake volume of only one uL, can easily be configured to run with either tubes or plates, and offers hands free performance of the staining, data acquisition and analysis.