Molecular Biology | School of Medicine

Azure 300

The Azure 300 is a highly versatile flatbed imager that can employ custom imaging options to produce high-resolution images of nucleic acid or protein gels, cultured cells, and slide-mounted samples. This instrument offers multiple wavelengths of illumination including broad band visible, UV, broad band blue, and broad band white light. The Azure 300 is most commonly used to image polyacrylamide or agarose gels tagged with primary and secondary antibodies of choice. Images generated using luminol can also be captured.

Bio-Plex 200

The Bio-Plex 200 is a multiplex immunoassay suspension array system that can simultaneously quantify concentrations of a wide range of biomarkers including cytokines, chemokines, growth factors, receptor and non-receptor proteins, nucleic acids and mRNA with up to 100 biomolecules per sample.

Bio-Rad CFX Opus 96

The Bio-Rad CFX Opus 96 is a real time PCR system that uses specialized 96 well plates for sample volumes of 10 to 50 µl. It offers excellent temperature accuracy and uniformity and can produce gradient runs in one degree C increments. Multiple options for digital connectivity and networking are also available.

LI-COR Odyssey CLx

The LI-COR Odyssey CLx is a dual wavelength infrared fluorescent imager used to detect and measure NIR fluorochrome labeled antibodies in immunoblotting assays. The Odyssey produces digital fluorescent images using infrared laser excitation and specialized optics for photon quantitation. The LI-COR Odyssey CLx can visualize faint and strong bands utilizing two different fluorescent excitation wavelengths (700 nm & 800 nm) with high signal to noise ratios, outstanding sensitivity and little or no image saturation. The Li-COR system includes proprietary software that facilitates efficient calibration and analysis of blotting results.

Nanodrop One spectrophotometer

The Nanodrop One spectrophotometer is a nanoscale spectrophotometer that can measure DNA, RNA and/or protein concentrations and purity in sample volumes of only 1 or 2 µl. The Nanodrop One is also capable of measurements at multiple fluorescent excitation/emission wavelength pairs for dye-based quantitation of nucleic acids. This instrument has an easy-to-use touchscreen interface to navigate between the different features and does not require a desktop tower.

Tecan Spark

The Tecan Spark reader offers an innovative combination of flexibility, ease of use and high throughput. It can be used for absorbance, fluorescence, luminescence, high performance laser excitation, brightfield imaging, automated cell imaging, and AlphaScreen bead-based proximity assays, all with strong environmental controls and excellent temperature stability. The Tecan Spark is also the first plate reader that can accommodate multimode live cell assays with whole well fluorescence imaging and flexible real-time experimental controls. This instrument can efficiently count nuclei, quantify GFP transfection efficiency, and can support assays that measure cell viability cell death, apoptosis and cell roughness.