Illumina HiSeq 4000 System
Illumina HiSeq 4000 System, purchased with the funds of a grant from the Ardmore Institute of Health (50%) and the donation from Dr. Charles A. Sims (50%), provides the highest data quality, cost-effective, and efficient sequencing for large-scale studies across multiple applications. From high-throughput gene expression profiling to deep transcriptional analysis, the HiSeq 4000 System offers unprecedented solutions for high-throughput RNA analysis. It can measure the abundance of individual transcripts and isoforms, discovery of new genes, and identification of regulatory non-coding RNAs. The HISeq 4000 System can sequence up to 100 human transcriptomes and 180 exomes per run. It can also enable large-scale whole-genome sequencing, making it feasible to sequence up to 12 whole human genomes per run with 30x coverage in less than 3.5 days. Furthermore, the HiSeq 4000 System can allow us to investigate the multiple forms of epigenetic regulations such as miRNA-Seq, ChIP-Seq, whole genome DNA methylation, Reduced Representation of Bisulfite Sequencing (RRBS), all at single base resolution.
Illumina NextSeq 550
Illumina NextSeq 550 System, funded by a NIH grant (PI, Wang), is an integrated system for automated generation of DNA clonal clusters by bridge amplification, sequencing, and primary data analysis. It is the first NGS system enabled for array scanning. By leveraging microarray scanning on the NextSeq 550, it is a powerful, complementary technology for further exploration or confirmation of copy number variants detected through sequencing. The system includes embedded touch screen monitor and on-instrument computer, providing the flexible power and simplicity. It is a wonderful, fast and also a powerful NGS sequencer. It can sequence up to 20 human transcriptomes in less than 11 hours (75 BPx1, >20M reads each) and one whole human genome in just 29 hours (150 BPx2) with 30x coverage.
The Illumina cBot System is a revolutionary automated system that creates clonal clusters from single molecule DNA templates, preparing them for sequencing by synthesis on the HiSeq sequencers. It allows for streamlined cluster generation and positive sample tracking, enabling clear and controlled sample identification through the sequencing workflow.
The MiSeqDx system advances our research further by being the first and only FDA-cleared in vitro diagnostic (IVD) next-generation sequencing (NGS) technology to target specific genetic variants and detect indels in homopolymeric regions with reliability and ease. The system shortens the path to clinical diagnosis by replacing multiple PCR steps increasing efficiency and providing more definitive results within less than 2 days. By reducing hands-on efforts, it minimizes human error, offers a small footprint, and eliminates the need for time-consuming additional testing with a simple three-step assay workflow.
10x Genomics Chromium Single Cell Controller
The Chromium Single Cell Controller system is a compact, sleek RNA sequencer for analyzing cell characterization and gene expression by profiling hundreds to millions of cells through automating the equivalency of 100,000s to 1,000,000 of pipetting steps for highly parallel sample partitioning and molecular barcoding. With a complete yet simplified workflow from cell suspension through sequencing-ready cDNA in less than a day, the Chromium Single Cell Controller ensures the cell capture efficiency up to 65%. This system is compatible with Illumina HiSeq4000/2500/NextSeq/MiSeq sequencers, which ensures a seamless transition from system to system.
Fluidigm C1™ Single-Cell Auto Prep System
The Fluidigm C1™ Single-Cell AutoPrep System is a new approach to single-cell gene expression. The system takes an entirely new approach, based on innovative microfluidic technology, to enables us to rapidly and reliably isolate, process and profile individual cells for genomic analysis such as single-cell RNA-Seq transcriptome measurement. For the first time, one can extract, reverse transcribe, pre-amplify and ultimately detect and analyze cell activity using just one technology, reducing the variability caused by multi-platform technical errors. The C1 Single-Cell AutoPrep System enables us to study cell differentiation, measure individual cell responses to specific stimuli, verify critical disease biomarkers, validate RNAi knockdown, and conduct candidate drug screens. With an optimized protocol, pre-formulated reagent kit, and disposable arrays, it can achieve single-cell accuracy with "load and go" productivity.
Life Technologies QuantStudio® 7 Real-Time PCR System
The Applied Biosystems® QuantStudio™ 7 Flex Real-Time PCR System advances our research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, intuitive software, automation capabilities, and touch-screen interface, the QuantStudio™ 7 Flex system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy. The system is compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
Qubit® 3.0 Fluorometer
The Qubit® 3.0 Fluorometer is the next generation of the popular benchtop fluorometer that accurately measures DNA, RNA, and protein using the highly sensitive Qubit® quantitation assays. The concentration of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants—including degraded DNA or RNA—on the result.
Agilent 2100 Bioanalyzer
The Agilent 2100 Bioanalyzer system can provide sizing, quantitation and quality control of DNA, RNA, DNA-Seq and RNA-Seq sequencing libraries, with high quality digital data.
Agilent 2200 TapeStation
The Agilent 2200 TapeStation system automates RNA and DNA sample QC, including sample loading, separation, and imaging. It is a tape-based platform for simpler, faster and more reliable electrophoresis for QC of DNA, RNA, DNA-Seq and RNA-Seq libraries
Covaris S220 Focused-ultrasonicator
The S220 Focused-ultrasonicator is a versatile, high power system engineered for pre-analytical sample processing. The versatility of the S220 Focused-ultrasonicator makes it possible to bring the advantages of AFA to numerous biological and chemical applications including, DNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization. It is an accurate ultrasonicator system for shearing the DNA and RNA to the desired and targeted sizes for DNA-Seq and RNA-Seq library constructions.
PEQLab Minilys Bead Mill Homogenizer
The PEQLab Minilys utilizes the same technology as the Precellys homogenizer range, which disrupts samples with a 3D, figure-8 motion. It efficiently disrupts samples in minutes and provides high yields and high quality of nucleic acids and proteins without sample vs. sample cross-contamination.
Sage Science PippinHT
PippinHT incorporates the Sage Science’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and other laboratories producing large-scale sequencing data.